Delbridge, Alex R. D. and Huh, Dann and Brickelmaier, Margot and Burns, Jeremy C. and Roberts, Chris and Challa, Ravi and Raymond, Naideline and Cullen, Patrick and Carlile, Thomas M. and Ennis, Katelin A. and Liu, Mei and Sun, Chao and Allaire, Normand E. and Foos, Marianna and Tsai, Hui-Hsin and Franchimont, Nathalie and Ransohoff, Richard M. and Butts, Cherie and Mingueneau, Michael (2020) Organotypic Brain Slice Culture Microglia Exhibit Molecular Similarity to Acutely-Isolated Adult Microglia and Provide a Platform to Study Neuroinflammation. Frontiers in Cellular Neuroscience, 14. ISSN 1662-5102
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Abstract
Microglia are central nervous system (CNS) resident immune cells that have been implicated in neuroinflammatory pathogenesis of a variety of neurological conditions. Their manifold context-dependent contributions to neuroinflammation are only beginning to be elucidated, which can be attributed in part to the challenges of studying microglia in vivo and the lack of tractable in vitro systems to study microglia function. Organotypic brain slice cultures offer a tissue-relevant context that enables the study of CNS resident cells and the analysis of brain slice microglial phenotypes has provided important insights, in particular into neuroprotective functions. Here we use RNA sequencing, direct digital quantification of gene expression with nCounter® technology and targeted analysis of individual microglial signature genes, to characterize brain slice microglia relative to acutely-isolated counterparts and 2-dimensional (2D) primary microglia cultures, a widely used in vitro surrogate. Analysis using single cell and population-based methods found brain slice microglia exhibited better preservation of canonical microglia markers and overall gene expression with stronger fidelity to acutely-isolated adult microglia, relative to in vitro cells. We characterized the dynamic phenotypic changes of brain slice microglia over time, after plating in culture. Mechanical damage associated with slice preparation prompted an initial period of inflammation, which resolved over time. Based on flow cytometry and gene expression profiling we identified the 2-week timepoint as optimal for investigation of microglia responses to exogenously-applied stimuli as exemplified by treatment-induced neuroinflammatory changes observed in microglia following LPS, TNF and GM-CSF addition to the culture medium. Altogether these findings indicate that brain slice cultures provide an experimental system superior to in vitro culture of microglia as a surrogate to investigate microglia functions, and the impact of soluble factors and cellular context on their physiology.
Item Type: | Article |
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Subjects: | OA Open Library > Medical Science |
Depositing User: | Unnamed user with email support@oaopenlibrary.com |
Date Deposited: | 19 May 2023 06:27 |
Last Modified: | 29 Jan 2024 05:49 |
URI: | http://archive.sdpublishers.com/id/eprint/810 |