Hyphal Formation is Enhanced by 121°C Heat Inactivation of Serum Added to Culture Media

He, Hong and Wang, Xueting and Li, Congcong and Deng, Xiaotong and Fan, Yan and Han, Jianxin (2020) Hyphal Formation is Enhanced by 121°C Heat Inactivation of Serum Added to Culture Media. Journal of Advances in Medicine and Medical Research, 32 (6). pp. 12-20. ISSN 2456-8899

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Official URL: https://doi.org/10.9734/jammr/2020/v32i630427

Abstract

Background: A novel method for inducing hyphae formation, was described.

Methods: Mycelia of experimental and clinical strains from 100 patients with oral lichen planus were cultured in RPMI 1640 medium with 56°C or 121°C inactivated calf sera. After these strains were cultured for 2 h to 7 days, the average hyphae formation rate was observed under light microscopy.

Results: The 121°C inactivated calf serum showed better outcomes than 56°C inactivated calf serum in terms of time and amounts of hyphae products.

Conclusions: For Candida albicans hyphae culture, the 121°C inactivated serum is superior to the 56°C inactivated serum.

Item Type:	Article
Subjects:	OA Open Library > Medical Science
Depositing User:	Unnamed user with email support@oaopenlibrary.com
Date Deposited:	05 Apr 2023 06:14
Last Modified:	20 Sep 2023 06:36
URI:	http://archive.sdpublishers.com/id/eprint/233

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