Callus Induction and Plant Regeneration in Sesame (Sesamum indicum L.)

Callus induction through hypocotyls or sub-hypocotyls or cotyledonary explants is routine in sesame (Sesamum indicumL.) where the explants are harvested after 8-10 days and were transferred on to callus induction media separately where callus proliferation was achieved in 15-35 days. But in a rapid method where callus was initiated on a media supplemented with different concentrations of BAP, Kinetin and NAA with direct seeding. Callus was initiated in a week's time from hypocotyls, cotyledonary leaves and roots concomitantly. The shoots that originate from the plumule of seeds were separated and subjected to direct organogenesis for multiple shoots induction. The callus developed can be harvested separately from three different sources (cotyledons, hypocotyls, and root) in a single seedling and can be used for shoot regeneration simultaneously. The time for callusing has considerably reduced (by 15-25 days). For instance, normal route of callusing will take 4-6 weeks, however, the new method established can save almost two-three weeks. It is hypothesized that this method should work for small seeded species.