In Vitro Propagation of Pergularia daemia (Forsk.) Chiov

The present study was conducted to establish a protocol for rapid propagation of Pergularia daemia (Asclepiadaceae) through different aseptic seedling explants. 40 days old aseptic seedlings grown on half strength MS medium were used as explant. All the explants were cultured on MS medium containing different concentration of cytokinins alone. MS medium supplemented with BAP 8.87 ?M produced maximum number of 11.20 shoots with the shoot tip explant. Nodal explant on BAP 13.3?M attained maximum shoot length of 4.78 cm. High frequency of rooting (85%) and maximum number of 6.2 roots were produced on half strength MS medium supplemented with IBA 0.49?M. After acclimatization, 70% of the micropropagated plantlets were successfully established in the field.