PREPARATION, CHARACTERIZATION AND STUDY OF THE BIOLOGICAL SCREENING OF THE AU (III) COMPLEX WITH NOVEL (AZO -SCHIFF BASE) LIGAND

The Aim: Biological studies carried out of new gold complexes after its preparation.

Place and Duration of Study: All preparations were carried out in college of education, chemistry lab between (Jan 2021 to May 2022).

Study Design: New azo –Schiff base ligand 2-amino-3-(2-((3-((2-hydroxy-4-sulfamoylphenyl)imino)-1,5- dimethyl- 2-phenyl- 2,3-dihydro- 1H-pyrazol-4-yl) diazenyl) -1H-imidazol-4-yl)propanoic acid with chelate complexes of Co(II),Ni(II), Cu(II), Zn(II), Cd(II) , Hg(II) and Au(III) were prepared with metal salts in ethanol a solvent in 1:1 ratio (metal : ligand) . The azo-Schiff base ligand has been synthesized from condensation of 2-amino-3- (2-((3-( (2 -hydroxy- 4-sulfamoylphenyl) imino)- 1,5-dimethyl -2-phenyl-2,3-dihydro-1H-pyrazol-4-yl)diazenyl)-1H-imidazol-4-yl)propanoic acid with 4-amino-3-hydroxybenzenesulfonamide.

Methodology: The prepared azo Schiff ligand and its metal complexes where checked by different spectral technique as, FT-IR ,C.H.N elemental analyses , Atomic absorption, magnetic moment measurements, molar conductance, UV-vis, ,1H-NMR and mass spectra studies.

Results: The data show that the complexes have the composition of 1:1[M:L] chelates type and octahedral geometry with each of Co(II), Ni(II),Cu(II),Zn(II), Cd(II) and Hg(II) ions, except for Au(III) ion which has square planer geometry. All complexes that non electrolytes and no conductive species but Au(III) complex is 1:2 electrolyte nature. This can support the electrolytic nature of the metal complex.

Conclusion: Biological activity studies of the ligand and their metal complexes against several organisms, bacteria Gram positive G(+ve)staphylococcus aureus and bacteria Gram negative G (- ve)Escherichia coli, In addition to fungi like Aspergillus Niger, and Aspergillus flavus , are reported. Compounds exhibited the high activity on the growth of all types microorganisms in this study . The results showed the highest inhibitory effect for gold the complex. The effect of biological screening of the gold complex on human colon cancer cell line HT- 29 was investigated. The gold complex was observed to have the highest inhibitory effect.