ANTIBIOFILM AND IN VITRO CYTOTOXIC ACTIVITY OF PIGMENT EXTRACTED FROM MARINE BACTERIA Kocuria flava (AP2)

Natural pigments can be obtained from various sources like plant, microorganisms, and marine algae because synthetic pigments have some toxicity issues and are carcinogenic to both products and workers. Therefore, it is thought that bacterial pigment is a crucial metabolic product beneficial to bacteria and may possess certain biological features like antioxidant, antibacterial, and anticancer activity. Therefore, the present study aimed to assess the antibiofilm and anticancer activities of crude pigment extract from a marine bacterial isolate. The Antibiofilm assay was studied by the 96-well plate method. The pigment from K. flava (AP2) has the ability to inhibit biofilm formation of Staphylococcus aureus, Streptococcus pyogenes, Serratia marcescens, Enterobacter sp, Enterococcus faecalis, and Escherichia coli. The optical density at 570 nm wavelength on a plate reader to quantify the overall bacterial growth in each well. Among the six strains, moderate biofilm inhibition were observed at 2.5 µg/ml. In-vitro anticancer activity of bacterial pigment extract was evaluated by MTT assay against human colon cancer (HT-29). As a result of the pigments' strong cytotoxic action, they could be employed as biological agents in medicine.