Development of Multivariate Calibration Technique for the Spectrophotometric Quantification of Ivermectin in Pharmaceutical Formulation

Objective: The current abstract employs a multivariate calibration technique to quantify ivermectin in pharmaceutical dosage form. Methods: The multivariate calibration technique relies on linear regression equations to correlate the relationship between concentration and absorbance at seven different wavelengths. The of ivermectin was found to be 254 nm. The findings were statistically analysed. This statistical approach yields the best results by eliminating fluctuations caused by instrumental or experimental conditions. Results: The developed method was validated in accordance with ICH guidelines and was found to be simple, linear, accurate, precise, and reproducible. The method was found to be linear over a concentration range of with a correlation coefficient value of about 0.9998. The limit of detection and quantification were found to be 0.029 and , respectively. Intraday and interday precision were found to have percentage relative standard deviations of 0.473-1.373 and 0.301-1.617, respectively. The percentage recovery was found within the range of . Conclusion: The findings show that a simple, linear, precise, accurate, sensitive, and reproducible multivariate calibration technique for the quantification of ivermectin in bulk and pharmaceutical formulation has been devised and validated.